diff --git a/pkgs/top-level/python-packages.nix b/pkgs/top-level/python-packages.nix
index 1ee24116ea14..701c5c6594e9 100644
--- a/pkgs/top-level/python-packages.nix
+++ b/pkgs/top-level/python-packages.nix
@@ -1934,12 +1934,6 @@ in {
     hypothesis = self.hypothesis.override { doCheck = false; };
   };
 
-  # Keep 3 because many test suites are not yet compatible with Pytest 4.
-  pytest_3 = callPackage ../development/python-modules/pytest/3.10.nix {
-    # hypothesis tests require pytest that causes dependency cycle
-    hypothesis = self.hypothesis.override { doCheck = false; };
-  };
-
   pytest-httpbin = callPackage ../development/python-modules/pytest-httpbin { };
 
   pytest-asyncio = callPackage ../development/python-modules/pytest-asyncio { };