diff --git a/pkgs/applications/science/biology/bowtie2/default.nix b/pkgs/applications/science/biology/bowtie2/default.nix
index 205ff9afdca1..818d0fe14ba2 100644
--- a/pkgs/applications/science/biology/bowtie2/default.nix
+++ b/pkgs/applications/science/biology/bowtie2/default.nix
@@ -1,4 +1,4 @@
-{ lib, stdenv, fetchFromGitHub, cmake, tbb, zlib }:
+{ lib, stdenv, fetchFromGitHub, cmake, tbb, zlib, python3, perl }:
 
 stdenv.mkDerivation rec {
   pname = "bowtie2";
@@ -13,7 +13,7 @@ stdenv.mkDerivation rec {
 
   nativeBuildInputs = [ cmake ];
 
-  buildInputs = [ tbb zlib ];
+  buildInputs = [ tbb zlib python3 perl ];
 
   meta = with lib; {
     description = "An ultrafast and memory-efficient tool for aligning sequencing reads to long reference sequences";